Journal Information
Vol. 83. Num. 4.October - December 2018
Pages 365-474
Visits
317
Vol. 83. Num. 4.October - December 2018
Pages 365-474
Original article
DOI: 10.1016/j.rgmxen.2018.06.003
Open Access
Is celiac disease better identified through HLA-DQ8 than through HLA-DQ2 in Mexican subjects?
¿Es posible una mejor identificación de la enfermedad celiaca en sujetos mexicanos por medio de HLA-DQ8 que de HLA-DQ2?
Visits
317
E. Cerda-Contrerasa, K.L. Ramírez-Cervantesa, J. Granadosb, L. Menab, C. Núñez-Álvarezc, L. Uscangaa,b,c,
Corresponding author
luis.uscangad@gmail.com

Corresponding author. Departamento de Gastroenterología. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Vasco de Quiroga 15. Tlalpan. 14000. Mexico City, Mexico. Tel.: +52 55 54 87 0900
a Department of Gastroenterology, Instituto Nacional de Ciencias Médicas y Nutrición «Salvador Zubirán», Mexico City, Mexico
b Department of Transplantation, Instituto Nacional de Ciencias Médicas y Nutrición «Salvador Zubirán», Mexico City, Mexico
c Department de Immunology, Instituto Nacional de Ciencias Médicas y Nutrición «Salvador Zubirán», Mexico City, Mexico
This item has received
317
Visits

Under a Creative Commons license
Article information
Abstract
Full Text
Bibliography
Download PDF
Statistics
Tables (1)
Table 1. Characteristics of the study subjects.
Abstract
Introduction and aims

A strong genetic association between celiac disease (CD) and the human leukocyte antigen (HLA) has been widely demonstrated. In Europe, the HLA-DQ2 allele is predominant. However, studies in Latin America indicate that HLA-DQ8 could be more frequent. In Mexico, the frequency of those alleles has not been reported in subjects with CD. Therefore, the aim of the present study was to evaluate the distribution of HLA-DQ2 and HLA-DQ8 in Mexican individuals with CD.

Material and methods

An exploratory study was conducted on a cohort of 49 subjects with chronic diarrhea. Autoantibodies for CD, duodenal atrophy, and HLA haplotypes were determined.

Results

Thirty individuals had CD (23 women, mean age 54.2 ± 15.5 years), 24 (80%) of whom expressed HLA-DQ8, 15 (50%) expressed HLA-DQ2, and 11 (37%) presented with both alleles. However, neither the HLA-DQ2 nor the HLA-DQ8 allele was found in 5 (10%) individuals. In subjects with chronic diarrhea that did not have CD, 12 (63%) presented with HLA-DQ2, and 7 (37%) with HLA-DQ8. Individuals with CD expressed the combinations of the HLA-DQ8/DQ2 alleles (37 vs. 5%) and the HLA-DR4/DQ8 alleles (60 vs. 26%) more frequently than the subjects without CD.

Conclusions

In Mexican subjects with CD, HLA-DQ8 distribution was more frequent than that of HLA-DQ2, indicating a possible similarity to the frequency reported in other Latin American countries. However, given the nature of the present study and its sample size, further conclusions could not be reached.

Keywords:
Celiac disease
HLA-DQ2
HLA-DQ8
Resumen
Introducción y objetivos

Existe una fuerte asociación entre la enfermedad celiaca (EC) y el antígeno leucocitario humano (HLA). En Europa predomina el alelo HLA-DQ2; sin embargo, estudios en América Latina indican que el HLA-DQ8 podría ser más frecuente. En México no se ha reportado la frecuencia de estos alelos en sujetos con EC. Por lo tanto, el objetivo de nuestro estudio fue determinar la distribución de HLA-DQ2 y HLA-DQ8 en sujetos mexicanos con EC.

Material y métodos

Se llevó a cabo un estudio exploratorio con una cohorte de 49 individuos, en quienes se buscó la presencia de marcadores serológicos, histológicos y genéticos de la EC.

Resultados

Treinta sujetos (23 mujeres) con una edad promedio de 54.2 ± 15.5 años presentaron EC; 24 (80%) de ellos expresaron HLA-DQ8 y 15 (50%) HLA-DQ2; 11 (37%) presentaron ambos alelos; sin embargo, en 5 (10%) individuos no se encontró HLA-DQ2 ni HLA-DQ8. Entre los sujetos con diarrea crónica que no tuvieron EC, 12 (63%) presentaron HLA-DQ2 y 7 (37%) HLA-DQ8. Los sujetos con EC expresaron más frecuentemente la combinación de HLA-DQ8/DQ2 (37% vs. 5%) y los alelos HLA-DR4/DQ8 (60% vs. 26%).

Conclusiones

En sujetos mexicanos con EC la expresión de HLA-DQ8 fue más frecuente que la de HLA-DQ2, lo cual indica que la distribución de HLA podría ser similar a las descritas en otros países de América Latina. Sin embargo, la naturaleza y el tamaño de la muestra de este estudio no permiten hacer más conclusiones.

Palabras clave:
Enfermedad celiaca
HLA-DQ2
HLA-DQ8
Full Text
Introduction and aims

Celiac disease (CD) is one of the most common food-related intestinal disorders. This condition is characterized by an abnormal immune response to the ingestion of gluten and related proteins in genetically susceptible individuals that have a polygenic disorder, with the participation of both human leukocyte antigen (HLA) genes and non-HLA genes.1 HLA-DQ2 and HLA-DQ8 are the most frequent, and the DQA1*05/DQB1*02 heterodimer is observed in 90-95% of the cases.2

In most European subjects with CD, HLA-DQ2 is more prevalent,3,4 but that same assumption remains controversial for Latin American populations. For instance, in a study conducted on pediatric and adult Amerindian subjects with CD, there was a higher frequency of HLA-DQ8.5–7 In addition, the authors of a population-based study carried out in different regions of Mexico found a distribution of 16% for HLA-DQ2 and 24% for HLA-DQ8,8 suggesting that the Mexican population could share some of the characteristics found in other Latin American countries.

The diagnostic criteria for CD include the combination of positive serologic auto-antibodies, such as anti-endomysium (EmA IgA), anti-transglutaminase (anti-tTG IgA/IgG), and anti-deamidated gliadin peptide (AGA-DGP IgA/IgG), together with the histologic demonstration of villous atrophy of the second part of the duodenum.9,10 However, in certain clinical situations, it is recommended to investigate the presence of HLA-DQ2/DQ8 to rule out the disease.9

In Mexico, the frequency of CD is similar to that described worldwide,11 but the distribution of HLA-DQ8/DQ2 in subjects with CD has not been reported. Therefore, the aim of the present study was to determine the distribution of the HLA-DQ2 and HLA-DQ8 alleles in Mexican subjects with CD.

Materials and methods

An exploratory study was conducted on 49 consecutive Mexican patients (38 women) with a mean age of 53 ± 14.5 years with chronic diarrhea (watery or loose stools for more than four weeks) and symptoms consistent with CD that were seen at the outpatient unit of a tertiary care referral center in Mexico City.

The study subjects underwent blood chemistry screening tests, serum levels of anti-tTG IgA/IgG and EmA IgA (INOVA Diagnostics, Inc., San Diego, CA, USA), stool analysis for parasites, and stool cultures. The individuals were also studied under the institutional protocol for chronic diarrhea, in which malabsorption is a serum beta-carotene level lower than 60μg/dl in females, and 50μg/dl in males, values consistently related to significant steatorrhea (stool fat 10g/d). In individuals with steatorrhea, a D-xylose absorption test in urine was performed (normal > 5g/vol).12

All the individuals had upper gastrointestinal endoscopies and duodenal aspirates, and at least four fragments of the second part of the duodenum were taken for analysis. Histologic findings were classified according to the Marsh-Oberhuber criteria,13 and CD was diagnosed through compatible intestinal biopsy histology (Marsh II-IV), and positive antibody tests (tTg-IgA/IgG/ EmA IgA). Small intestinal bacterial overgrowth (SIBO) was defined when bacterial populations in the small intestine exceeded 105-106 organisms/ml.14

Class II antigens (HLA-DRB1, HLA-DQB1) were typed by the PCR-SSP procedure (Pel-Freez HLAA/B/DR/DQ SSP Unity, Brown Deer, Wisconsin, USA), and the HLA-DQ8 allele was determined as a generic category (broad antigen).

A Mexican mestizo was defined as a person born in Mexico, whose last two ascending generations were also born in Mexico. Demographic data were collected, and the patients were classified into two groups: group A, patients with celiac disease and group B, patients with chronic diarrhea from other causes. The physicians that classified the patients were blinded to the results of the HLA analysis.

All participants signed written statements of informed consent and the study was approved by the Institutional Review Board for Biomedical Studies on Humans Beings (ref. 1719).

Statistical analysis

The distribution of the HLA-DQ2 and HLA-DQ8 alleles was reported. The frequencies were compared between patients with CD and those with chronic diarrhea from other causes, but no statistical comparisons were performed between the 2 groups.

Results

The demographic characteristics are shown in Table 1. The diagnosis of CD was established in 30 subjects (23 women) with a mean age of 54.2 ± 15.5 years. In the rest of the individuals, SIBO was the most common diagnosis (15 women), mean age 50.7 ± 12.5 years).

Table 1.

Characteristics of the study subjects.

Age, years (mean ± SD)  53 ± 14.5 
Female sex  38 (78%) 
BMI (mean ± SD)  21 ± 4.6 
Diagnosisa
CD  30 (61%) 
SIBO  10 (20%) 
Autoimmune enteropathy  2 (4%) 
Lymphocytic colitis  2 (4%) 
Pancreatic insufficiency  2 (4%) 
Intestinal tuberculosis  1 (2%) 
IBS-D  1 (2%) 
Without diagnosis  5 (10%) 

CD: Celiac disease; IBS-D: Irritable bowel syndrome-diarrhea; SIBO: Small intestinal bacterial overgrowth.

a

Some patients had more than one diagnosis.

The expression of HLA-DQ2 was similar between the 2 study groups (50% vs. 63%), but HLA-DQ8 was more frequent in patients with CD (80% vs. 37%). The frequency of HLA-DQ8 persisted after including the prevalence of HLA-DR4/DQ8 haplotype, and the combination of both HLA-DQ2 and HLA-DQ8 was more frequent in subjects with CD (37% vs. 5%).

Discussion and conclusions

The present study is the first to measure the frequency and distribution of the HLA-DQ2 and HLA-DQ8 alleles in a Mexican population with CD. Our results are similar to observations made worldwide, regarding the high frequency of the HLA-DQ2 and HLA-DQ8 haplotypes in subjects with CD. However, unlike most of the European populations, the subjects with CD from our study expressed the HLA-DQ8 haplotype more frequently. That same association was previously described in other South American individuals with CD,6,15 coinciding with the high prevalence of HLA-DQ8 previously reported in Mexican mestizo subjects.16 On the other hand, the frequency of the HLA-DQ8 haplotype was interestingly low in individuals with chronic diarrhea without CD, which was also the case when the DR4/DQ8 haplotype presented. In general, an unexpectedly high prevalence of HLA-DQ2 was found, being even higher than the frequency of HLA-DQ2 reported by Barquera et al.8 in the general Mexican population.

The most frequent diagnosis in the group of chronic diarrhea from other causes was SIBO, and subjects became asymptomatic after receiving specific treatment.14–17 In 5 (10%) cases no diagnosis was established, and seronegative CD was ruled out because its presence was not supported by histologic findings or response to a gluten-free diet.

Regarding the limitations of the present study, the small sample size did not allow stronger assumptions to be made. However, ours is the first exploratory research analyzing the distribution of the HLA-DQ2 and HLA-DQ8 alleles in Mexican individuals with CD. It is also the first report to suggest that the HLA-DQ8 allele and DR4/DQ8 haplotype could be more prevalent than HLA-DQ2 in Mexican individuals with CD, supporting the findings of other Latin American populations. Finally, it is important to emphasize that, given the nature of the present study and its small sample size, the results obtained must be further evaluated.

Ethical disclosuresProtection of human and animal subjects

The authors declare that no experiments were performed on humans or animals for this study.

Confidentiality of data

The authors declare that no patient data appear in this article.

Right to privacy and informed consent

The authors declare that no patient data appear in this article.

Financial disclosure

No financial support was received in relation to this study/article.

Conflict of interest

The authors declare that there is no conflict of interest.

References
[1]
J. Ludvigsson, D. Leffler, J. Bai
The Oslo definitions for coeliac disease and related terms
[2]
J. Romanos, C. van Diemen, I. Nolte
Analysis of HLA and non-HLA Alleles can identify individuals at high risk for celiac disease
Gastroenterology., 137 (2009), pp. 834-840 http://dx.doi.org/10.1053/j.gastro.2009.05.040
[3]
M. Limongelli, M. Bourgey, O. Esposito
HLA-related genetic risk for coeliac disease
Dig Liver Dis., 39 (2007), pp. A64
[4]
P. Margaritte-Jeannin, M.C. Babron, M. Bourgey
HLA-DQ relative risks for coeliac disease in European populations: A study of the European Genetics Cluster on Coeliac Disease
Tissue Antigens., 63 (2004), pp. 562-567 http://dx.doi.org/10.1111/j.0001-2815.2004.00237.x
[5]
T.C. Johnson, B. Diamond, L. Memeo
Relationship of HLA-DQ8 and severity of celiac disease: Comparison of New York and Parisian cohorts
Clin Gastroenterol Hepatol, 2 (2004), pp. 888-894
[6]
F. Pérez-Bravo, M. Araya, A. Mondragón
Genetic differences in HLA-DQA1* and DQB1* allelic distributions between celiac and control children in Santiago, Chile
Hum Immunol, 60 (1999), pp. 262-267
[7]
M. Araya, A. Mondragón, F. Pérez-Bravo
Celiac disease in a Chilean population carrying Amerindian traits
J Pediatr Gastroenterol Nutr, 31 (2000), pp. 381-386
[8]
R. Barquera, J. Zuñiga, R. Hernández-Díaz
HLA class i and class ii haplotypes in admixed families from several regions of México
Mol Immunol, 45 (2008), pp. 1171-1178 http://dx.doi.org/10.1016/j.molimm.2007.07.042
[9]
A. Rubio-Tapia, I.D. Hill, C.P. Kelly
American College of G, ACG clinical guidelines: Diagnosis and management of celiac disease
Am J Gastroenterol, 108 (2013), pp. 656-676 http://dx.doi.org/10.1038/ajg.2013.79
[10]
V. Abadie, L.M. Sollid, L.B. Barreiro
Integration of genetic and immunological insights into a model of celiac disease pathogenesis
[11]
J. Remes-Troche, C. Nuñez-Alvares, L. Uscanga-Domínguez
Celiac disease in Mexican population: An update
Am J Gastroenterol., 108 (2013), pp. 283-284 http://dx.doi.org/10.1038/ajg.2012.408
[12]
E. Galvan-Guerra, T. Ramírez-Iglesias, G. Robles-Díaz
Diagnostic utility of serum beta-carotenes in intestinal malabsorption syndrome
Rev Invest Clin, 46 (1994), pp. 99-104
[13]
G. Oberhuber, G. Granditsch, H. Vogelsang
The histopathology of coeliac disease: Time for a standardized report scheme for pathologists
Eur J Gastroenterol Hepatol, 11 (1999), pp. 1185-1194
[14]
A. Rubio-Tapia, S.H. Barton, J.E. Rosenblatt
Prevalence of small intestine bacterial overgrowth diagnosed by quantitative culture of intestinal aspirate in celiac disease
J Clin Gastroenterol, 43 (2009), pp. 157-161 http://dx.doi.org/10.1097/MCG.0b013e3181557e67
[15]
A. Parada, M. Araya, F. Pérez-Bravo
Amerindian mtDNA haplogroups and celiac disease risk HLA haplotypes in mixed-blood Latin American patients
J Pediatr Gastroenterol Nutr, 53 (2011), pp. 429-434 http://dx.doi.org/10.1097/MPG.0b013e31821de3fc
[16]
G. Vargas-Alarcón, J. Granados, J.M. Rodríguez-Pérez
Distribution of HLA class II alleles and haplotypes in Mexican Mestizo population: Comparison with other populations
Immunol Invest, 39 (2010), pp. 268-283 http://dx.doi.org/10.3109/08820131003681151
[17]
M.S. Chang, P.H. Green
A review of rifaximin and bacterial overgrowth in poorly responsive celiac disease
Therap Adv Gastroenterol, 5 (2012), pp. 31-36 http://dx.doi.org/10.1177/1756283X11422264

Please cite this article as: Cerda-Contreras E, Ramírez-Cervantes KL, Granados J, Mena L, Núñez-Álvarez C, Uscanga L. ¿Es posible una mejor identificación de la enfermedad celiaca en sujetos mexicanos por medio de HLA-DQ8 que de HLA-DQ2? Revista de Gastroenterología de México. 2018;83:410–413.

Idiomas
Revista de Gastroenterología de México

Subscribe to our Newsletter

Article options
Tools
es en
Política de cookies Cookies policy
Utilizamos cookies propias y de terceros para mejorar nuestros servicios y mostrarle publicidad relacionada con sus preferencias mediante el análisis de sus hábitos de navegación. Si continua navegando, consideramos que acepta su uso. Puede cambiar la configuración u obtener más información aquí. To improve our services and products, we use "cookies" (own or third parties authorized) to show advertising related to client preferences through the analyses of navigation customer behavior. Continuing navigation will be considered as acceptance of this use. You can change the settings or obtain more information by clicking here.